Synthesis and characterization of a lipase-friendly DES based on cholinium dihydrogen phosphate

The main objective of the present work was to synthesize and characterize three novel lipase-friendly deep eutectic solvents (DESs) using cholinium dihydrogen phosphate (N1112OHDHP) as hydrogen bond acceptor ethylene glycol (EG) and/or glycerol (GL) donors in following proportions: N1112OHDHP:EG (molar ratio 1:2), N1112OHDHP:GL 1:2) N1112OHDHP:EG:GL 1:1:1). FTIR, 1H NMR, TGA DSC were employed shed light on their distinctive structural characteristics. Density, refractive index dynamic viscosity also measured at different temperatures, experimental data correlated with several well-known equations. Finally, suitability newly synthesized DESs be biocatalytic reactions demonstrated by analysing activity a commercial enzyme (Candida antarctica lipase B) after being contact them.